Hopefully, Alli could only miss the game with Slovenia at Wembley next Thursday when a win will secure the Three Lions' place for Russian Federation 2018.
"We run the risk of looking a shambles because he could get withdrawn again", England manager Gareth Southgate said.
Alli could be suspended for up to four competitive matches for making an offensive gesture during England's 2-1 win over Slovakia at Wembley last month.
Watford midfielder Nathaniel Chalobah, Arsenal striker Danny Welbeck and Burnley goalkeeper Tom Heaton - members of the last squad - miss out through injury.
"Our hope is that common sense prevails and he is available for our matches, but we'll have to wait and see", he added.
The Manchester City midfielder has not played worldwide football since a 2-0 defeat to Spain in November 2015.
"I have had a good chat with Dele".
Elsewhere in the squad, Southampton goalkeeper Fraser Forster is also included, coming in for Burnley's Tom Heaton who is now sidelined with a shoulder injury. "We hope to have him available".
Southgate omitted a number of injured players, including Jamie Vardy, while the likes of Theo Walcott and Luke Shaw, among others, have not convinced the coach of a recall following their unimpressive start to the new campaign.
England lead Group F on 20 points, five points ahead of second-placed Slovakia, and are one win away from sealing their spot in the 2018 World Cup in Russian Federation.